Table 1.

Bacterial strains used in this study

StrainRelevant genotypeSource or reference
S. typhimurium
 ATCC 14028-1swt1a American Type Culture Collection
 SR-11wt1a R. Curtiss III
 LB5010 metA22 metE551 ilv-452 leu-3121 trpD2 xyl-404 galE856 hsdLT6 hsdSA29 hsdSB121 rpsL120 12
 146 polA-2 zig214::Tn10(Tetr)M. Rhen
 SF1005 rpoS::pRR10(ΔtrfA)(Ampr)D. Guiney
 UMR1ATCC 14028-1s Nalr This study
 UMR3SR-11 Nalr This study
 MAE1UMR1 ΔcsgA101::Kmr This study
 MAE2UMR3 ΔcsgA101::Kmr This study
 MAE5UMR1 ΔcsgA101 This study
 INK1SR-11zcg-101::Kmr This study
 MAE40SF1005 × UMR1; UMR1rpoS::pRR10(ΔtrfA)(Ampr)This study
 MAE29SF1005 × SR-11; SR-11 rpoS::pRR10 (ΔtrfA)(Ampr)This study
 MAE46UMR1 ΔompR101::Ampr This study
 MAE34UMR3 ΔompR101::Ampr This study
E. coli K-12
 DH5α endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1Δ(lacZYA-argF)U169(φ80lacZΔM15)Laboratory collection
 MC4100F araD139Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR Laboratory collection
 XL1 Blue MRΔ(mcrA)183Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Stratagene
 HLO7MC4100 Δ(csgG-csgC)::Kmr 47a
 MHR204MC4100 csgA2::Tn105Cmr 34
 MHR210MC4100csgG1::Tn105Cmr 34
 MHR261MC4100csgB2 35
 MHR426MC4100csgF4 36
 MHR480MC4100 ΔcsgE3 37
 MHR503MC4100csgD6 This study
  • a wt1, wild type 1.