Table 2.

Plasmids used and constructed in this study

PlasmidCharacterizationReference
pLAFR3TetrRK2 oriT64
pMAK700Cmrtemperature-sensitive replicon derived from pSU10133
pMAK705pMAK700 containing pUC19 polylinker33
pRK2013KmrColE1 mob tra27
pUC4KAmpr Kmr cassettePharmacia
pWSK29Ampr pSC101ori67
pWKS30AmprpSC101 ori67
pUMR2bpMAK700 ΔcsgA101; CmrThis study
pUMR2c-1pMAK700 ΔcsgA101::Kmr; CmrKmrThis study
pUMR10-7pBS,NsiI-BamHI-cut CSGD1-CSGB3 from SR-11; Ampr56a
pMU1pUC18,PstI fragment of zcg-101; AmprThis study
pMU2pUC18,HindIII-PstI fragment of zcg-101; AmprThis study
pMU3apMAK705,HindIII-PstI fragment ofzcg-101; CmrThis study
pMU3b-1pMAK705, zcg-101::Km; Cmr KmrThis study
pMU4pLAFR3 containing a 39-kb PstI fragment harboring thecsg genes; Tetr KmrThis study
pCSGApWKS30, PstI-BamHI-cut CSGB2-CSGC; AmprThis study
pCSGBpWKS30,HindIII-PstI-cut CSGBD-CSGA1; AmprThis study
pCSGDpWSK29 (SmaI/EcoRI), EcoRI-cut SP2-SP5; AmprThis study
pCSGEpWSK29 (SmaI/BamHI), BstYI-cut SP5-SP9; AmprThis study
pCSGFpWKS30 (EcoRI/SmaI), EcoRI-cut SP6-SP17; AmprThis study
pCSGEFGpWSK29 (PstI/EcoRI), NsiI-cut CSGD2-SP21; AmprThis study
pUMR7b-2pMAK705 ΔompR101::Ampr; CmrAmprThis study