Table 2.

Plasmids used and constructed in this study

PlasmidCharacterizationReference
pLAFR3TetrRK2 oriT 64
pMAK700Cmrtemperature-sensitive replicon derived from pSU101 33
pMAK705pMAK700 containing pUC19 polylinker 33
pRK2013KmrColE1 mob tra 27
pUC4KAmpr Kmr cassettePharmacia
pWSK29Ampr pSC101ori 67
pWKS30AmprpSC101 ori 67
pUMR2bpMAK700 ΔcsgA101; Cmr This study
pUMR2c-1pMAK700 ΔcsgA101::Kmr; CmrKmr This study
pUMR10-7pBS,NsiI-BamHI-cut CSGD1-CSGB3 from SR-11; Ampr 56a
pMU1pUC18,PstI fragment of zcg-101; Ampr This study
pMU2pUC18,HindIII-PstI fragment of zcg-101; Ampr This study
pMU3apMAK705,HindIII-PstI fragment ofzcg-101; Cmr This study
pMU3b-1pMAK705, zcg-101::Km; Cmr Kmr This study
pMU4pLAFR3 containing a 39-kb PstI fragment harboring thecsg genes; Tetr Kmr This study
pCSGApWKS30, PstI-BamHI-cut CSGB2-CSGC; Ampr This study
pCSGBpWKS30,HindIII-PstI-cut CSGBD-CSGA1; Ampr This study
pCSGDpWSK29 (SmaI/EcoRI), EcoRI-cut SP2-SP5; Ampr This study
pCSGEpWSK29 (SmaI/BamHI), BstYI-cut SP5-SP9; Ampr This study
pCSGFpWKS30 (EcoRI/SmaI), EcoRI-cut SP6-SP17; Ampr This study
pCSGEFGpWSK29 (PstI/EcoRI), NsiI-cut CSGD2-SP21; Ampr This study
pUMR7b-2pMAK705 ΔompR101::Ampr; CmrAmpr This study