Table 3.

Citrate lyase assays in E. coli/pBluescript and E. coli harboring the citDEFG cluster

StrainCitrate lyase activity (U/mg)
Initial activityAfter treatment with acetic anhydrideIn the presence of acetyl-CoA (5 mM)
BL21/pBluescript KS+0.0510.0600.059
BL21/pNA20280.0730.0850.162