Table 3.

Substrate specificity of enoyl-CoA hydratase encoded by phaJAc

SubstrateKm(μM)Vmax (U/mg)
Crotonyl-CoA296.2 × 103
2-Pentenoyl-CoA362.8 × 103
2-Hexenoyl-CoA341.8 × 103
2-Octenoyl-CoA502.5