Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference(s)
A. caviae
 FA440Wild typeFERM P-3432;8, 26
 AC004PHA-negative mutant of FA440This study
E. coli
 DH5αdeoR endA1 gyrA96 hsdR17(rK mK+)recA1 relA1 supE44 thi-1 Δ(lacZYA-argFV169) φ80 ΔlacZΔM15 FrClontech
 S17-1recA andtra genes of plasmid RP4 integrated into chromosome; auxotrophic for proline and thiamine27
 BL21(DE3)FompT hsdSB(rBmB) gal dcm (DE3)Novagen
pJRD215Cosmid; KmrSmr RSF1010 replicon; Mob+5
pJRDEE323.2-kbp insert (EcoRI fragment containing phaCAc, ORF1, and ORF3 [phaJAc]) in pJRD21511
pJRDEE32d12.8-kbp insert (EcoRI fragment containing phaCAc and ORF3 [phaJAc]) in pJRD21511
pJRDEE32d32.8-kbp insert (EcoRI fragment containing phaCAc and ORF1) in pJRD21511
pJRDEE32d132.4-kbp insert (EcoRI fragment containingphaCAc) in pJRD21511
pJRDG131.4-kbp insert (EcoRI fragment containing ORF1 and ORF3 [phaJAc]) in pJRD215This study
pEE323.2-kbp insert (EcoRI fragment containingphaCAc, ORF1, and ORF3 [phaJAc]) in pUC1811
pET-3aApr; contains a T7 promoter with start codon and designed ribosome binding siteNovagen
pETNB30.4-kbp insert (PCR-amplifiedNdeI-BamHI fragment of ORF3 [phaJAc]) in pET-3aThis study