TABLE 2.

Quorum-repressed genes

Gene no.aDescriptionMaximum changeb
lasI rhlI mutantWild type vs lasR rhlR mutant
3OC12-HSLC4-HSL + 3OC12-HSL
PA0165Hypothetical protein−2.7−2.9 (2.0)−4.8 (2.0)
PA0433Hypothetical protein−6.8−20 (2.0)−8.9 (1.4)
PA0434Hypothetical protein−7.7−8.5 (2.0)−5.6 (2.0)
PA0435Hypothetical protein−9.4−26 (2.0)−34 (2.0)
PA0485Conserved hypothetical proteinc−1.7−3.4 (1.4)−3.0 (3.0)
PA0887acsA, acetyl-coenzyme A synthetase−3.3−4.2 (2.0)−3.6 (3.0)
PA1559Hypothetical protein−2.4−3.5 (2.0)−3.2 (1.4)
PA2007maiA, maleylacetoacetate isomerase−3.2−1.4 (4.0)−3.2 (3.0)
PA2008fahA, fumarylacetoacetase−3.7−1.5 (4.0)−2.6 (3.0)
PA2009hmgA, homogentisate 1,2-dioxygenase−4.0−1.5 (4.0)−2.7 (3.0)
PA2250lpdV, lipoamide dehydrogenase-Val−3.1−1.8 (4.0)−2.6 (3.0)
PA2338Probable component of ABC maltose transporter−5.0−3.2 (3.0)−4.2 (3.0)
PA2339Probable maltose-mannitol transport protein−1.9−6.8 (3.0)−4.1 (3.0)
PA2340Probable maltose-mannitol transport protein−3.4−2.0 (3.0)−3.7 (3.0)
PA2341Probable component of ABC maltose transporter−3.1−2.0 (3.0)−4.2 (3.0)
PA2343mtlY, xylulose kinase−1.7−4.0 (3.0)−3.2 (4.0)
PA3038Probable porin−2.3−3.5 (2.0)−4.4 (3.0)
PA3174Probable transcriptional regulator−2.1−3.5 (4.0)−6.5 (3.0)
PA3205Hypothetical protein−1.3−3.1 (4.0)−3.1 (4.0)
PA3233Hypothetical protein−2.2−2.7 (3.0)−5.1 (3.0)
PA3234Probable sodium-solute symporter−4.5−3.4 (2.0)−7.0 (3.0)
PA3235Conserved hypothetical protein−3.9−4.2 (3.0)−6.6 (3.0)
PA3281Hypothetical protein−5.7−6.4 (1.4)−25 (1.4)
PA3282Hypothetical protein−8.5−8.8 (1.4)−21 (1.4)
PA3283Conserved hypothetical protein−9.0−8.8 (1.4)−28 (1.4)
PA3284Hypothetical protein−7.1−10 (2.0)−24 (1.4)
PA3364amiC, aliphatic amidase expression-regulating protein−2.7−1.8 (4.0)−2.7 (1.4)
PA3365Probable chaperone−3.0−1.7 (4.0)−4.0 (1.4)
PA3575Hypothetical protein−1.6−2.7 (1.4)−3.3 (2.0)
PA3790oprC, outer membrane protein OprC−2.7−3.7 (2.0)−4.6 (2.0)
PA4359Conserved hypothetical protein−1.4−2.7 (2.0)−2.8 (1.4)
PA4371Hypothetical protein−1.9−4.1 (2.0)−2.8 (1.4)
PA4442cysN, ATP sulfurylase GTP-binding subunit−2.8−3.4 (3.0)−7.6 (2.0)
PA4443cysD, ATP sulfurylase small subunit−3.1−3.4 (3.0)−6.5 (2.0)
PA4691Hypothetical protein−2.5−2.8 (2.0)−2.9 (2.0)
PA4692Conserved hypothetical protein−3.8−3.4 (2.0)−5.0 (1.4)
PA4770IldP, l-lactate permease−1.8−3.7 (2.0)−5.0 (2.0)
PA5168Probable dicarboxylate transporter−2.7−1.9 (4.0)−5.8 (2.0)
  • a Gene number from the Pseudomonas genome project (http://www.pseudomonas.com).

  • b Maximum changes in gene expression (rounded to two significant figures) in the signal generation mutant in the presence of the signal(s) indicated compared with the absence of signal and in the wild-type P. aeruginosa strain compared with the receptor mutant. The values in parentheses are the OD600 at which the earliest change of ≥2.5-fold was observed (for the signal generation mutant both time courses were considered).

  • c There is a las-rhl box-like sequence with an HI of ≥10 and <13.