TABLE 2.

Quorum-repressed genes

Gene no.aDescriptionMaximum changeb
lasI rhlI mutantWild type vs lasR rhlR mutant
3OC12-HSLC4-HSL + 3OC12-HSL
PA0165Hypothetical protein−2.7−2.9 (2.0)−4.8 (2.0)
PA0433Hypothetical protein−6.8−20 (2.0)−8.9 (1.4)
PA0434Hypothetical protein−7.7−8.5 (2.0)−5.6 (2.0)
PA0435Hypothetical protein−9.4−26 (2.0)−34 (2.0)
PA0485Conserved hypothetical proteinc−1.7−3.4 (1.4)−3.0 (3.0)
PA0887 acsA, acetyl-coenzyme A synthetase−3.3−4.2 (2.0)−3.6 (3.0)
PA1559Hypothetical protein−2.4−3.5 (2.0)−3.2 (1.4)
PA2007 maiA, maleylacetoacetate isomerase−3.2−1.4 (4.0)−3.2 (3.0)
PA2008 fahA, fumarylacetoacetase−3.7−1.5 (4.0)−2.6 (3.0)
PA2009 hmgA, homogentisate 1,2-dioxygenase−4.0−1.5 (4.0)−2.7 (3.0)
PA2250 lpdV, lipoamide dehydrogenase-Val−3.1−1.8 (4.0)−2.6 (3.0)
PA2338Probable component of ABC maltose transporter−5.0−3.2 (3.0)−4.2 (3.0)
PA2339Probable maltose-mannitol transport protein−1.9−6.8 (3.0)−4.1 (3.0)
PA2340Probable maltose-mannitol transport protein−3.4−2.0 (3.0)−3.7 (3.0)
PA2341Probable component of ABC maltose transporter−3.1−2.0 (3.0)−4.2 (3.0)
PA2343 mtlY, xylulose kinase−1.7−4.0 (3.0)−3.2 (4.0)
PA3038Probable porin−2.3−3.5 (2.0)−4.4 (3.0)
PA3174Probable transcriptional regulator−2.1−3.5 (4.0)−6.5 (3.0)
PA3205Hypothetical protein−1.3−3.1 (4.0)−3.1 (4.0)
PA3233Hypothetical protein−2.2−2.7 (3.0)−5.1 (3.0)
PA3234Probable sodium-solute symporter−4.5−3.4 (2.0)−7.0 (3.0)
PA3235Conserved hypothetical protein−3.9−4.2 (3.0)−6.6 (3.0)
PA3281Hypothetical protein−5.7−6.4 (1.4)−25 (1.4)
PA3282Hypothetical protein−8.5−8.8 (1.4)−21 (1.4)
PA3283Conserved hypothetical protein−9.0−8.8 (1.4)−28 (1.4)
PA3284Hypothetical protein−7.1−10 (2.0)−24 (1.4)
PA3364 amiC, aliphatic amidase expression-regulating protein−2.7−1.8 (4.0)−2.7 (1.4)
PA3365Probable chaperone−3.0−1.7 (4.0)−4.0 (1.4)
PA3575Hypothetical protein−1.6−2.7 (1.4)−3.3 (2.0)
PA3790 oprC, outer membrane protein OprC−2.7−3.7 (2.0)−4.6 (2.0)
PA4359Conserved hypothetical protein−1.4−2.7 (2.0)−2.8 (1.4)
PA4371Hypothetical protein−1.9−4.1 (2.0)−2.8 (1.4)
PA4442 cysN, ATP sulfurylase GTP-binding subunit−2.8−3.4 (3.0)−7.6 (2.0)
PA4443 cysD, ATP sulfurylase small subunit−3.1−3.4 (3.0)−6.5 (2.0)
PA4691Hypothetical protein−2.5−2.8 (2.0)−2.9 (2.0)
PA4692Conserved hypothetical protein−3.8−3.4 (2.0)−5.0 (1.4)
PA4770 IldP, l-lactate permease−1.8−3.7 (2.0)−5.0 (2.0)
PA5168Probable dicarboxylate transporter−2.7−1.9 (4.0)−5.8 (2.0)
  • a Gene number from the Pseudomonas genome project (http://www.pseudomonas.com).

  • b Maximum changes in gene expression (rounded to two significant figures) in the signal generation mutant in the presence of the signal(s) indicated compared with the absence of signal and in the wild-type P. aeruginosa strain compared with the receptor mutant. The values in parentheses are the OD600 at which the earliest change of ≥2.5-fold was observed (for the signal generation mutant both time courses were considered).

  • c There is a las-rhl box-like sequence with an HI of ≥10 and <13.