TABLE 2.

Kinetic parameters for autophosphorylation of the PhoQ cytoplasmic domain in the presence of increasing amounts of acetyl-CoAa

Acetyl-CoA (mM)Km (μM)kcat (min−1)kcat/Km (M−1 min−1)
055 ± 140.0026 ± 0.001147 ± 18
1.2552 ± 40.0018 ± 0.000834 ± 15
1.7549 ± 170.0008 ± 0.000317 ± 0.2
2.5058 ± 190.0002 ± 0.00014.0 ± 3.0
  • a Autokinase assays were performed at 22°C in 10 mM Tris-HCl (pH 8.0)-25 mM KCl-0.4 mM MgCl2. Kinetic parameters were derived as described in Materials and Methods. Mean values and standard deviations were calculated from at least three independent experiments.