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functional genomics

  • Role of <em>epaQ</em>, a Previously Uncharacterized <span class="named-content genus-species" id="named-content-1">Enterococcus faecalis</span> Gene, in Biofilm Development and Antimicrobial Resistance
    Special Issue Meeting Presentation
    Role of epaQ, a Previously Uncharacterized Enterococcus faecalis Gene, in Biofilm Development and Antimicrobial Resistance

    E. faecalis is a common cause of nosocomial infection, has a high level of antibiotic resistance, and forms robust biofilms. Biofilm formation is associated with increased antibiotic resistance. Therefore, a thorough understanding of biofilm-associated antibiotic resistance is important for combating resistance. Several genes from the epa operon have...

    Michelle L. Korir, Jennifer L. Dale, Gary M. Dunny
  • Open Access
    Guanidine Riboswitch-Regulated Efflux Transporters Protect Bacteria against Ionic Liquid Toxicity
    Research Article | Spotlight
    Guanidine Riboswitch-Regulated Efflux Transporters Protect Bacteria against Ionic Liquid Toxicity

    This study identifies bacteria that are tolerant to ionic liquid solvents used in the production of biofuels and industrial biochemicals. For industrial microbiology, it is essential to find less-harmful reagents and microbes that are resistant to their cytotoxic effects. We identified a family of small multidrug resistance efflux transporters, which are responsible for the tolerance of these strains. We also found that this resistance...

    Douglas A. Higgins, John M. Gladden, Jeff A. Kimbrel, Blake A. Simmons, Steven W. Singer, Michael P. Thelen
  • Characterization of <span class="sc">l</span>-Carnitine Metabolism in <span class="named-content genus-species" id="named-content-1">Sinorhizobium meliloti</span>
    Research Article
    Characterization of l-Carnitine Metabolism in Sinorhizobium meliloti

    The experimental characterization of novel metabolic pathways is essential for realizing the value of genome sequences and improving our knowledge of the enzymatic capabilities of the bacterial world. However, 30% to 40% of genes of a typical genome remain unannotated or associated with a putative function. We used enzyme kinetics, liquid chromatography-mass spectroscopy (LC-MS)-based metabolomics, and mutant phenotyping for the...

    Pascal Bazire, Nadia Perchat, Ekaterina Darii, Christophe Lechaplais, Marcel Salanoubat, Alain Perret
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