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RNA polymerase

  • The Context-Dependent Influence of Promoter Sequence Motifs on Transcription Initiation Kinetics and Regulation
    Minireview
    The Context-Dependent Influence of Promoter Sequence Motifs on Transcription Initiation Kinetics and Regulation

    The fitness of an individual bacterial cell is highly dependent upon the temporal tuning of gene expression levels when subjected to different environmental cues. Kinetic regulation of transcription initiation is a key step in modulating the levels of transcribed genes to promote bacterial survival.

    Drake Jensen, Eric A. Galburt
  • VpsR Directly Activates Transcription of Multiple Biofilm Genes in <span class="named-content genus-species" id="named-content-1">Vibrio cholerae</span>
    Research Article
    VpsR Directly Activates Transcription of Multiple Biofilm Genes in Vibrio cholerae

    Vibrio cholerae, the bacterial pathogen that is responsible for the disease cholera, uses biofilms to aid in survival, dissemination, and persistence. VpsR, which directly senses the second messenger c-di-GMP, is a major regulator of this process. Together with c-di-GMP, VpsR directly activates transcription by RNA polymerase containing σ70 from the ...

    Meng-Lun Hsieh, Christopher M. Waters, Deborah M. Hinton
  • The Core and Holoenzyme Forms of RNA Polymerase from <em>Mycobacterium smegmatis</em>
    Research Article
    The Core and Holoenzyme Forms of RNA Polymerase from Mycobacterium smegmatis

    We describe here three-dimensional structures of core and holoenzyme forms of mycobacterial RNA polymerase (RNAP) solved by cryo-electron microscopy. These structures fill the thus-far-empty spots in the gallery of the pivotal forms of mycobacterial RNAP and illuminate the extent of conformational dynamics of this enzyme. The presented findings may facilitate future designs of antimycobacterial drugs targeting RNAP.

    Tomáš Kouba, Jiří Pospíšil, Jarmila Hnilicová, Hana Šanderová, Ivan Barvík, Libor Krásný
  • Research Article
    Association of ω with the C-Terminal Region of the β′ Subunit Is Essential for Assembly of RNA Polymerase in Mycobacterium tuberculosis
    Chunyou Mao, Yan Zhu, Pei Lu, Lipeng Feng, Shiyun Chen, Yangbo Hu
  • Research Article
    A Bacteriophage Capsid Protein Is an Inhibitor of a Conserved Transcription Terminator of Various Bacterial Pathogens
    Gairika Ghosh, Jayavardhana Reddy, Susmit Sambhare, Ranjan Sen
  • Research Article
    An Amino Acid Substitution in RNA Polymerase That Inhibits the Utilization of an Alternative Sigma Factor
    Anna F. Wang Erickson, Padraig Deighan, Cinthia P. Garcia, Robert O. J. Weinzierl, Ann Hochschild, Richard Losick
  • Research Article
    Exploring the Amino Acid Residue Requirements of the RNA Polymerase (RNAP) α Subunit C-Terminal Domain for Productive Interaction between Spx and RNAP of Bacillus subtilis
    Cierra A. Birch, Madison J. Davis, Lea Mbengi, Peter Zuber
  • Research Article | Spotlight
    Effects of Increasing the Affinity of CarD for RNA Polymerase on Mycobacterium tuberculosis Growth, rRNA Transcription, and Virulence
    Ashley L. Garner, Jayan Rammohan, Jeremy P. Huynh, Lucas M. Onder, James Chen, Brian Bae, Drake Jensen, Leslie A. Weiss, Ana Ruiz Manzano, Seth A. Darst, Elizabeth A. Campbell, Bryce E. Nickels, Eric A. Galburt, Christina L. Stallings
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